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A novel hyaluronic acid-based dissolving microneedle patch loaded with ginsenoside Rg3 liposome for effectively alleviate psoriasis
Abstract
Psoriasis is a chronic scaly skin disease, which is associated with abnormal immune system function, inflammatory cell infiltration. The disadvantages of traditional treatment of psoriasis are poor efficacy, recurrence of the condition, long treatment time, and systemic side effects due to a lack of an appropriate drug delivery system. A liposome-in-Microneedles (MNs) based transdermal drug delivery system had been developed to provide efficient site-specific drug delivery, stable drug release and enhanced permeability. Firstly, liposomes were loaded with Ginsenoside Rg3 (a small molecular compound with anti-inflammatory and immune regulation functions), then Rg3-liposomes (Rg3-Lipo) were successfully loaded into MNs. Rg3-Liposome-MNs (Rg3-MNs) with the mechanical property of 0.59 N/needle, could easily penetrate the epidermis and release Rg3-lipo to regulate the immune response. The efficacy of Rg3-MNs was evaluated by IMQ-induced psoriasis-like dermatitis in mice. Rg3-MNs significantly (p < 0.01) decreased the epidermal thickness, dermal papillary edema, and the possible mechanism was to markedly reduce (p < 0.01) the levels of IL-17, IL-23, and TNF-α. In addition, we found that Rg3-MNs might improve psoriasis by inhibiting the activation of the STAT3/p-STAT3 signaling pathway. In brief, this study provides an ideal loaded anti-inflammatory and immunoregulatory drug delivery system to treat psoriasis.
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2. Materials and methods
2.1. Materials
Ginsenoside 20(S)-Rg3 was purchased from Chengdu DeSiTeBiological Technology Co., ltd. (Chengdu, China).
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Supplementary data to this article can be found online at https://doi.org/10.1016/j.matdes.2022.111363.
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CircSV2b participates in oxidative stress regulation through miR-5107-5p-Foxk1-Akt1 axis in Parkinson's disease
As a novel type of non-coding RNAs, covalently closed circular RNAs (circRNAs) are ubiquitously expressed in eukaryotes. Emerging studies have indicated that dysregulation of circRNAs was related to neurological diseases. However, the biogenesis, regulation, function, and mechanism of circRNAs in Parkinson's disease (PD) remain largely unclear. In this study, thirty-three differentially expressed circRNAs (DECs) were detected by RNA-sequencing between the MPTP-induced PD mice model and the wild-type mice. Quantitative real-time PCR was used to determine the RNA level of DECs in the striatum (STR), substantia nigra pars compacta (SNpc), and serum exosomes, and it was found that circSV2b was downregulated in PD mice. Then, functional experiments in vivo were employed to explore the effect of circSV2b in PD. For the mechanism study, dual-luciferase reporter, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP),RNA pull-down, gene editing, and CUT & Tag were performed in vitro to confirm that circSV2b directly sponged miR-5107-5p and alleviated the suppression of the expression of the target gene Foxk1, and then positively regulated Akt1 transcription.In vivo, the mechanistic analysis demonstrated that circSV2b overexpression resisted oxidative stress damage through the ceRNA-Akt1 axis in PD models. Taken together,these findings suggested that the miR-5107-5p-Foxk1-Akt1 axis might serve as a key target of circSV2b overexpression in PD treatment, and highlighted the significant change of circSV2b in serum exosomes. Therefore, circSV2b might be a novel biomarker for the diagnosis and treatment of PD.
Keywords: circSV2b, miR-5107-5p, Foxk1, Akt1, oxidative stress
High Pressure Liquid Chromatography (HPLC) Detection
Mice in each group were sacrificed and their brains were rapidly removed......To quantify the sample peaks, each chemical was compared to external standards (DA hydrochloride, DD0177; DOPAC, DE0034; HVA, DG0021. Desite, Chengdu, China)
文献地址：https://doi.org/10.1016/j.redox.2022.102430
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Journal of separation science-03 March 2019
Ionic liquid vortex-simplified matrix solid-phase dispersion for the simultaneous determination of terpenoids, crocins, quinic acid derivatives and flavonoids in Gardeniae fructus by UHPLC期刊名：Journal of separation science文献编号：DOI 10.1002/jssc.201801354文献地址：https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201801354发表日期：03 March 2019AbstractA novel ionic‐liquid‐based vortex‐simplified matrix solid‐phase dispersion method using 2,6‐dimethyl‐β‐cyclodextrin was established by ultra high performance liquid chromatography coupled with a photodiode array detector. 2,6‐Dimethyl‐β‐cyclodextrin was first used as a promising adsorbent in this proposed method for simultaneous determination of eight compounds in Gardeniae fructus. These compounds are terpenoids (geniposidic acid, genipin‐1‐β‐D‐gentiobioside, geniposide, 8‐o‐acetyl shanzhiside methyl ester), crocins (crocin‐I, crocin‐II), quinic acid derivatives (chlorogenic acid), and flavonoids (isoquercitrin), respectively. Several parameters were investigated in the adsorption and desorption processes to obtain the optimal conditions, including 2,6‐dimethyl‐β‐cyclodextrin as sorbent, 0.5 mL 100 mM 1‐dodecyl‐3‐methylimidazolium hydrogen sulfate as the extraction solvent, 2:1 of sample/sorbent ratio, grinding for 2 min and vortexing for 60 s. The recoveries of the eight compounds ranged from 96.6 to 100% (<3.50%). The limits of detection and quantification were in the range of 0.02–0.30 and 0.06–1.25 μg/mL, respectively. Meanwhile, a good linearity was attained with r values (>0.9997). The established method showed higher extraction efficiency and less reagent consumption than traditional matrix solid phase dispersion and ultrasonic‐assisted extraction. Hence, it could be applied for sample preparation and analysis of natural products.Standard substances including geniposidic acid, chlorogenic acid, genipin-1-β-Dgentiobioside, geniposide, 8-o-acetyshanzhiside methyl ester, isoquercitrin, crocin I, and crocin II were purchased from Chengdu Desite Biological Technology Co., Ltd(Chengdu, China).
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Frontiers in pharmacology-09 January 2019
Simultaneous Extraction and Determination of Compounds With Different Polarities From Platycladi Cacumen by AQ C18-Based Vortex-Homogenized Matrix Solid-Phase Dispersion With Ionic Liquid期刊名：Frontiers in pharmacology文献编号：DOI 10.3389/fphar.2018.01532文献地址：https://link.springer.com/article/10.1007%2Fs13318-017-0444-8发表日期：09 January 2019 This study presented a rapid, simple and environmentally friendly method of employing AQ C18-based vortex-homogenized matrix solid-phase dispersion with ionic liquid (AQ C18-IL-VHMSPD) for the extraction of compounds with different polarities from Platycladi Cacumen (PC) samples by ultra high-performance liquid chromatography with PDA detection. AQ C18 (aqua C18) and ionic liquid ([Bmim]BF4) were used as the adsorbent and green elution reagent in vortex-homogenized MSPD procedure. The AQ C18-IL-VHMSPD conditions were optimized by studying several experimental parameters including the type of ionic liquid, the type of adsorbent, ratio of sample to adsorbent, the concentration and volume of ionic liquid, grinding time and vortex time. The recoveries of the target compounds were in the range of 96.9–104% with relative standard deviation values no more than 2.8%. The limits of detection and limits of quantitation were in the range of 0.2–1.2 and 1.0–5.4 ng mL-1, respectively. Compared with the traditional ultrasonic-assisted extraction, the developed AQ C18-IL-VHMSPD method required less sample, reagent and time. It was concluded that the AQ C18-IL-VHMSPD method was a powerful method for the extraction and quantification of the high polarity and low polarity compounds in traditional Chinese medicines samples.Reference standards of myricitrin, isoquercitrin, quercitrin,amentoflavone and hinokiflavone were purchased from Chengdu Desite Biological Technology Co., Ltd (Chengdu, China)
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