Simultaneous determination of calycosin, prim-O glucosylcimifugin, and paeoniflorin in rat plasma by HPLC-MS/MS: application in the pharmacokinetic analysis of HQCF

This study aimed to develop and validate a high-performance liquid chromatography– 
tandem mass spectrometry method to simultaneously determine three bioactive components of 
the Huangqi Chifeng decoction (HQCF) in rat plasma. 
Methods: Taxol was used as an internal standard in the developed method. Chromatographic 
separation was performed on a C18 column using a gradient elution with 0.1% formic acid in 
acetonitrile (v/v) and 0.1% formic acid in water (v/v) as the mobile phases at a flow rate of 
0.4 mL
minute1 . All compounds were monitored via selected reaction monitoring with an 
electrospray ionization source. 
Results: The lower limits of quantification of paeoniflorin, calycosin, and prim-O-glucosylcimifu
gin were 15.0, 0.75, and 0.75 ng
mL1 , respectively. The calibration curves indicated optimal 
linearity (r > 0.99) across the concentration ranges. The specificity, precision, accuracy, recovery, 
matrix effect, and stability of the method were validated. This method was successfully applied in 
a pharmacokinetics study of the three compounds in rat plasma.

......

CA, PG, PA, and Taxol were purchased from Chengdu DeSiTe Biological Technology Co. Ltd. (Chengdu, China). 

原文链接：https://doi.org/10.1177/0300060520972902