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Journal of Chromatography A
Contents lists available at ScienceDirect
journal homepage: www.elsevier.com/locate/chroma
Published: 27 April 2021
Abstract
Due to the tremendous clinical value, more and more Traditional Chinese Medicines (TCMs) and their。formulae are attracted by world’s attention. QiangHuoShengShi (QHSS) decoction is one of classic TCM。formulae, which is clinically used for treating various rheumatic diseases. However, the phytochemical。constituents of QHSS have rarely been reported. A simple, intelligent, and comprehensive strategy was。developed to characterize the phytochemical-fingerprint and quantify the chemical-markers for precise。quality evaluation of QHSS. Firstly, a new deep-learning assisted mass defect filter (MDF) method was built for rapid and accurate classification of mass spectrum (MS) ions acquired by ultra-high performanceliquid chromatography quadrupole time of flight tandem mass spectrometry (UHPLC-Q-TOF/MS). Subse-quently, herb species-specific chemical-category and characteristic identification were used for further characterization of multi-components. As the result, seven major types of compounds in QHSS were in-telligently differentiated and 183 phytochemical compounds were tentatively identified. Finally, a sensi-tive scheduled multiple reaction monitoring (sMRM) detection method was applied to precisely quantify 37 target analytes in QHSS decoction. This integrated strategy would provide an alternative method for chemical-material basis study of more herbal medicine or natural products.
Authentic standards including protocatechuic acid, neochloro-genic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid,umbelliferone, scopoletin, prim-O-glucosylcimifugin, ferulic acid,agnuside, 10-O-vanilloylaucubin, liquiritin, isoferulic acid, liquir-itin apioside, cimifugin, 4´-O-β-D-glucosyl-5-O-methylvisamminol,isochlorogenic acid C, isoliquiritin apioside, isoliquiritin, liquiritigenin, ononin, formononetin, columbianetin, oxypeucedanin hy-drate, sec-O-glucosylhamaudol, psoralen, isoliquiritigenin, casticin,senkyunolide A, notopterol, imperatorin, phellopterin, neocnidilide,ligustilide, osthole, isoimperatorin, columbianadin, and glycyrrhizicacid were purchased from Chengdu Desite Bio-Technology Co., Ltd(Chengdu, China). The purity of the above standards was higherthan 98%.
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Frontiers in Pharmacology-08 March 2019
A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography期刊名：Frontiers in Pharmacology文献编号：DOI 10.3389/fphar.2019.00227文献地址：https://www.frontiersin.org/articles/10.3389/fphar.2019.00227/full发表日期：08 March 2019A simple and eco-friendly Diol-based-matrix solid-phase dispersion method (MSPD) was optimized and established to simultaneously extract 13 bioactive compounds (7 coumarins and 6 phenolic acids) in Angelicae Pubescentis Radix (APR) by ultrahigh performance liquid chromatography coupled with photodiode array detector (UHPLC-PDA). Diol was chosen as the dispersing sorbent and methanol solution was used as the elution solvent. The preparation procedures for the MSPD including the types of sorbents, mass ratio of matrix to sorbent, grinding time, type, concentration and volume of elution solvent were investigated. Under the optimal conditions, good recoveries of the 13 target compounds were obtained in the range of 94.8–107% (RSD < 3.22%). The limits of detection (LODs) and limits of quantitation (LOQs) were in the ranges of 0.08–0.12 μg mL-1 and 0.16–0.24 μg mL-1, respectively. Compared with the traditional method, it was a green and environmentally friendly technique. The results proved that the established method was successfully applied to the extraction and determination of 13 target bioactive compounds for quality control in APR.neochlorogenic acid, chlorogenic acid, 4-dicaffeoylquinic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C,umbelliferae, columbianetin, imperatorin, osthole, isoimperatorin were obtained from Chengdu Desite Biological Technology Co., Ltd. (Chengdu, China).
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Frontiers in pharmacology-17 June 2020 geniposidic acid
Standard substances such as geniposidic acid, neochlorogenic acid, chlorogenic acid, caffeic acid, geniposide, genipin, pinoresinol di-o-glucopyranoside, syringaresinol di-o-glucopyranoside, isochlorogenic acid A, pinoresinol o-glucopyranoside, and isochlorogenic acid C were purchased from Chengdu Desite Bio-Technology Co., Ltd (Chengdu, China).
原文地址：https://www.frontiersin.org/articles/10.3389/fphar.2020.00838/full
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Frontiers in pharmacology-17 June 2020
期刊名：Frontiers in pharmacology文献编号：https://doi.org/10.3389/fphar.2020.00838文献地址：https://www.frontiersin.org/articles/10.3389/fphar.2020.00838/full发表日期：17 June 2020Standard substances such as geniposidic acid, neochlorogenic acid, chlorogenic acid, caffeic acid, geniposide, genipin, pinoresinol di-o-glucopyranoside, syringaresinol di-o-glucopyranoside, isochlorogenic acid A, pinoresinol o-glucopyranoside, and isochlorogenic acid C were purchased from Chengdu Desite Bio-Technology Co., Ltd (Chengdu, China).
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Caffeic Acid, an Allelochemical in Artemisia argyi……
Artemisia argyi is widely distributed in Asia, and it often becomes the dominant population in the field because of its strong ecological niche competitiveness. Allelochemicals secreted by plants are generally considered an important reason for their dominance in ecological competition. In this study, the allelochemicals in A. argyi were screened by a series of experiments and their mechanisms were explored via transcriptomics. First, the inhibitory effects of A. argyi on Echinochloa crusgalli, Setaria viridis, Portulaca oleracea and Amaranthus retroflexus were evaluated. Then, we carried out a qualitative and quantitative analysis of the chemical composition of the aqueous extract of A. argyi to screen for potential allelochemicals that can inhibit weed growth. Four potential allelochemicals were quantified: neochlorogenic acid (5-CQA), chlorogenic acid (3-CQA), cryptochlorogenic acid (4-CQA), and caffeic acid (CA). …………
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Materials and Methods
Plant Materials and Chemicals
……Neochlorogenic acid (5-CQA, 99.6%), chlorogenic acid (3-CQA, 99.5%), cryptochlorogenic acid (4-CQA, 98.7%), and caffeic acid (CA, 99.7%) were provided by Chengdu Desite Bio-Technology Co., Ltd. (Chengdu, China). …………
原文链接：https://doi.org/10.3389/fpls.2021.802198
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