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Journal of Ethnopharmacology
Contents lists available at ScienceDirect
https://doi.org/10.1016/j.jep.2021.114336
Received 25 January 2021; Received in revised form 2 June 2021; Accepted 11 June 2021
ABSTRACT
Ethnopharmacological relevance: Viticis fructus (VF) has been widely used in alleviating the swelling and pain, owning to its pharmacologically active components including agnuside,10-O-vanilloylaucubin, luteolin and casticin.Aim of the study: The pharmacokinetic profiles of the absorbed components from aqueous and ethanolic ex-tracts of VF in rat plasma were performed, and explored the molecular mechanisms of absorbed components via network pharmacology.
Materials and methods: Ultra-performance liquid chromatography-tandem mass spectroscopy (UHPLC-MS/MS)was employed to identify the absorbed components from rat plasma. Liquid-liquid extraction with ethyl acetate was used to purify the plasma samples. Plasma pharmacokinetics parameters of the components absorbed were analyzed after oral administration of both extracts. Network pharmacology was used to predict the biological functions and potential signaling pathways of VF. The anti-cancer effects of VF extract and absorbed components have been confirmed by in vitro experiments.
Results: The method was very sensitive with lower limit of quantification (LLOQ) of 1.0, 2.5, 0.2 and 0.5 ng/mL for agnuside, 10-O-vanilloylaucubin, luteolin and casticin, respectively. With the exception of 10-O-vanil-loylaucubin which was not detected in the ethanolic extract of VF, all other components were detected in both extracts in plasma. The pharmacokinetic parameters of the four components from rat plasma were significantly different between the two extracts. According to the results of network pharmacology, the absorption compo-nents of VF are enriched in 32 key pathways, and 15 pathways are related to cancer. Ultimately, the anti-cancer effects, as well as the signaling pathways of VF ethanolic extract and absorbed components were veri-fied by in vitro experiments.
Conclusion: The optimized, sensitive and validated UHPLC-MS/MS method was successfully applied for the plasma pharmacokinetics comparison analysis of the two VF extracts. The combination of network pharma-cology and pharmacokinetics provides a useful method to elucidate the biological effects and molecular mechanism of the absorbed components of VF.
laboratory. Agnuside, luteolin, casticin, formononetin and geniposide (purity on HPLC> 98%) were supplied by Chengdu Desite Bio-Technology (Chengdu, China).
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Journal of separation science-03 March 2019
Ionic liquid vortex-simplified matrix solid-phase dispersion for the simultaneous determination of terpenoids, crocins, quinic acid derivatives and flavonoids in Gardeniae fructus by UHPLC期刊名：Journal of separation science文献编号：DOI 10.1002/jssc.201801354文献地址：https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201801354发表日期：03 March 2019AbstractA novel ionic‐liquid‐based vortex‐simplified matrix solid‐phase dispersion method using 2,6‐dimethyl‐β‐cyclodextrin was established by ultra high performance liquid chromatography coupled with a photodiode array detector. 2,6‐Dimethyl‐β‐cyclodextrin was first used as a promising adsorbent in this proposed method for simultaneous determination of eight compounds in Gardeniae fructus. These compounds are terpenoids (geniposidic acid, genipin‐1‐β‐D‐gentiobioside, geniposide, 8‐o‐acetyl shanzhiside methyl ester), crocins (crocin‐I, crocin‐II), quinic acid derivatives (chlorogenic acid), and flavonoids (isoquercitrin), respectively. Several parameters were investigated in the adsorption and desorption processes to obtain the optimal conditions, including 2,6‐dimethyl‐β‐cyclodextrin as sorbent, 0.5 mL 100 mM 1‐dodecyl‐3‐methylimidazolium hydrogen sulfate as the extraction solvent, 2:1 of sample/sorbent ratio, grinding for 2 min and vortexing for 60 s. The recoveries of the eight compounds ranged from 96.6 to 100% (<3.50%). The limits of detection and quantification were in the range of 0.02–0.30 and 0.06–1.25 μg/mL, respectively. Meanwhile, a good linearity was attained with r values (>0.9997). The established method showed higher extraction efficiency and less reagent consumption than traditional matrix solid phase dispersion and ultrasonic‐assisted extraction. Hence, it could be applied for sample preparation and analysis of natural products.Standard substances including geniposidic acid, chlorogenic acid, genipin-1-β-Dgentiobioside, geniposide, 8-o-acetyshanzhiside methyl ester, isoquercitrin, crocin I, and crocin II were purchased from Chengdu Desite Biological Technology Co., Ltd(Chengdu, China).
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Frontiers in pharmacology-17 June 2020 geniposidic acid
Standard substances such as geniposidic acid, neochlorogenic acid, chlorogenic acid, caffeic acid, geniposide, genipin, pinoresinol di-o-glucopyranoside, syringaresinol di-o-glucopyranoside, isochlorogenic acid A, pinoresinol o-glucopyranoside, and isochlorogenic acid C were purchased from Chengdu Desite Bio-Technology Co., Ltd (Chengdu, China).
原文地址：https://www.frontiersin.org/articles/10.3389/fphar.2020.00838/full
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Frontiers in pharmacology-17 June 2020
期刊名：Frontiers in pharmacology文献编号：https://doi.org/10.3389/fphar.2020.00838文献地址：https://www.frontiersin.org/articles/10.3389/fphar.2020.00838/full发表日期：17 June 2020Standard substances such as geniposidic acid, neochlorogenic acid, chlorogenic acid, caffeic acid, geniposide, genipin, pinoresinol di-o-glucopyranoside, syringaresinol di-o-glucopyranoside, isochlorogenic acid A, pinoresinol o-glucopyranoside, and isochlorogenic acid C were purchased from Chengdu Desite Bio-Technology Co., Ltd (Chengdu, China).
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