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Journal of Ethnopharmacology (Volume 260, 5 October 2020, 113044)
https://doi.org/10.1016/j.jep.2020.113044Received 16 March 2020; Received in revised form 11 May 2020; Accepted 28 May 2020Available online 11 June 2020
Abstract
Ethnopharmacological relevance: San-Ye-Tang-Zhi-Qing formula (SYTZQ) is an effffective prescription for the treatment of pre-diabetes disorders of glycolipid metabolism in type 2 diabetes mellitus (T2DM). It consists of five Chinese herbs including Mori Folium, Nelumbinis Folium, Crataegi Folium, Salviae Miltiorrhizae Radix et Rhizoma and Paeoniae Radix Rubra.
Aim of the study: This study was aimed to reveal the pharmacological mechanism of pharmacokinetic target components of SYTZQ for the treatment of T2DM.
Materials and methods: A rapid, precise and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to quantify simultaneously nuciferin, vitexin-4″-O-glu-coside, vitexin-2″-O-rhamnoside, paeoniflflorin and rosmarinic acid in rat plasma after oral administration of SYTZQ. The network pharmacology was used to analyze the effffect of the compounds absorbed into the blood of SYTZQ on T2DM. The effffects of paeoniflflorin, nuciferine and rosmarinic acid on adipogenic difffferentiation werevalidated in vitro experiments.
Results: The separation was performed on an ACQUITY UHPLC HSS T3 column (2.1 mm × 100 mm, 1.7 μm) using acetonitrile and 0.1% (v/v) formic acid in water as the mobile phase in gradient elution. The calibration curves of fifive analytes showed good linearity (r ≥ 0.9991) with the lower limits of quantifification (LLOQ) between 0.3 and 5.0 ng/mL. The recoveries and matrix effffects of fifive analytes ranged from 81.1% to 113%. The RSDs of inter-day and intra-day precision were all within 13.7%. The validated method was successfully appliedto the pharmacokinetic study of fifive ingredients after oral administration of SYTZQ to rat. 39 major targets and22 candidate pathways of fifive compounds absorbed into the blood of rats after administration of SYTZQ were identifified and successfully constructed a compound-target-disease-pathway network. It was confifirmed that paeniforin, nuciferine and rosmarinic acid could decrease the adipogenicity difffferentiation in vitro experiments. Conclusions: The pharmacokinetic parameters indicated that the fifive components (nuciferin, vitexin-4″-O-glu-coside, vitexin-2″-O-rhamnoside, paeoniflflorin and rosmarinic acid) were absorbed and eliminated quickly in vivo. These fifive absorbed components were associated with 22 pathways, including insulin resistance, regulation of lipolysis in adipocytes, PI3k/AKT-, TNF-, cAMP- and cGMP-PKG-signaling pathway. Paeoniflflorin, nuciferine and rosmarinic acid have the effffect of inhibiting adipocyte difffferentiation. This study could provide more re-ference for quality control, and provide a fifirm basis for evaluating the clinical effiffifficiency of SYTZQ.
Nuciferin, vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, paeoni-flflorin, rosmarinic acid, genipin (IS1), berberine (IS2) and baicalein (IS3) were purchased from Chengdu Desite Biotechnology Co., Ltd.(Chengdu, China). All purity of these ingredients was more than 98%.The chemical structures of those ingredients were presented in Fig. 1.
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Journal of Separation Science-03 February 2018
Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid-phase dispersion extraction coupled with ultra high-performance liquid chromatography with photodiode array detection and quadrupole time-of-flight mass spectrometry期刊名：Journal of Separation Science文献编号：DOI 10.1002/jssc.201701411文献地址：https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201701411发表日期：03 February 2018
Abstract
A simple and effective sample preparation process based on miniaturized matrix solid‐phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5‐dicaffeoylqunic acid, 1,5‐dicaffeoylqunic acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol‐3‐O‐rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenanthraquinones (cryptotanshinone), and furans (5‐hydroxymethylfurfural) in Naoxintong capsule by ultra high‐performance liquid chromatography. The optimized condition was that 25 mg Naoxintong powder was blended homogeneously with 100 mg Florisil PR for 4 min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid‐phase dispersion coupled with ultra high‐performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid‐phase dispersion coupled with ultra high‐performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.Reference standards including gallic acid (GA), 5-hydroxymethylfurfural, chlorogenic acid, paeoniflorin, ferulic acid, 3,5-dicaffeoylqunic acid, 1,5-dicaffeoylqunic acid, kaempferol-3-O-rutinoside, rosmarinic acid, lithospermic acid, salvianolic acid B, calycosin, formononetin, ligustilide, butyllidephthalide, and cryptotanshinone were purchased from Chengdu Desite Biological Technology Co., Ltd (Chengdu, China)
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A multi-evaluating strategy for Weikangling capsules: ……
Abstract
Weikangling capsules (WKLCs), a Chinese patent medicine consisting of 8 Chinese drugs, have been widely used in clinic to treat gastrointestinal diseases for more than 30 years. The current “Chinese Pharmacopoeia” (2020 Edition, ChP2020) uses paeoniflorin content (≥ 1.0 mg per capsule) as the standard of quality control, but it is insufficient to evaluate the overall quality of WKLCs. An efficient and economic method for quality control is urgently needed to ensure the quality consistency and clinical effects of WKLCs. Herein, a systematic and reliable method for the rapid analysis of chemical components in WKLCs was established for the first time based on ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). A total of 115 components covering 7 herbs in WKLCs were preliminarily identified by comparison with standard substances or literature. To evaluate the quality of 26 batches of WKLCs, a new method of fingerprinting combined with quantitative analysis was established, and 16 common peaks were selected to establish the fingerprint similarity model (similarity>0.90). Simultaneously, the contents of albiflorin, paeoniflorin, dactylorhin A, militarine, and glycyrrhizic acid were determined to be 0.82 ± 0.22, 2.09 ± 0.24, 1.15 ± 0.40, 3.73 ± 0.76 and 0.99 ± 0.20 mg/capsule, respectively. The transfer rates and dissolution curves of the five compounds were successfully detected in WKLCs, and the average transfer rates were 67.2%, 33.0%, 68.3%, 54.7%, and 33.7%, respectively. Notably, the dissolution profiles of different manufacturers presented remarkable differences in pH 1.2 hydrochloric acid solution. This method not only qualitatively identified the chemical components of Chinese patent medicines at the microlevel but also evaluated the quality consistency between batches at the macrolevel, which provided a comprehensive reference for the quality consistency of Chinese patent medicines between batches.
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2. Materials and methods
2.1. Chemicals and materials
…… The detailed information of all samples is listed in Table S1. The standard substances, including liquiritin, isoliquiritin, liquiritin apioside, isoliquiritin apioside, ononin, gly-cyrrhizic acid, glycyrrhetinic acid, paeoniflorin, albiflorin, oxypaeo-niflorin, benzoylpaeoniflorin, militarine, corydaline, tetrahydropalmatine, protopine, ginsenoside Rg1, ginsenoside Rb1, and ginsenoside Re were purchased from Chengdu Desite Biological Technology Co., Ltd. (Sichuan, China);
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原文链接：https://doi.org/10.1016/j.jpba.2021.114347
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