Effects of HSYA on serum and brain cholesterol levels....

Abstract    Hydroxysafflor yellow A (HSYA) has a certain improvement effect on Alzheimer's disease (AD) rats, but its specific mechanism is still unclear. The purpose of this study was to observe the regulatory effect of HSYA on learning and memory ability of AD rats induced by Aβ1-42. To explore the effective targets and potential  molecular mechanisms of HSYA in AD treatment based on quantitative proteomics.  Through the Morris water maze experiment, we found that after HSYA treatment, the learning ability of rats in the model group has been significantly improved.  Quantitative proteomics results showed that among the 11 common differential  proteins between the "model/sham operation" comparison group and the "HSYA  treatment/model" comparison group, the cholesterol synthesis rate-limiting enzyme mevalonate decarboxylase (Mvd) Western Blot results are consistent with the results of quantitative proteomics analysis.We found that HSYA can inhibit the expression of BACE protein in hippocampus of AD rats and decrease the level of Aβ1-42. Besides, HSYA could also reduce cholesterol levels in serum and hippocampus. In summary, HSYA can effectively improve learning and memory disorders in AD rats, and exert neuroprotective effects by effectively controlling serum and brain cholesterol to down-regulate the expression of BACE and thus reduce the content of Aβ1-42.   ………… HSYA (purity > 98%，Fig. 1a) was purchased from Chengdu DeSiTe Biological Accepted ManuscriptTechnology Co., Ltd. (Sichuan, China); 600mg HSYA was dissolved in 200ml double distilled water and prepared into 3 mg/mL HSYA reserve solution and stored at 4℃. The dose of HSYA was 30mg/kg, and the volume of HSYA was calculated according to body weight …………   原文链接：https://doi.org/10.1080/00207454.2022.2082964