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Frontiers in Pharmacology-08 March 2019
A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography期刊名：Frontiers in Pharmacology文献编号：DOI 10.3389/fphar.2019.00227文献地址：https://www.frontiersin.org/articles/10.3389/fphar.2019.00227/full发表日期：08 March 2019A simple and eco-friendly Diol-based-matrix solid-phase dispersion method (MSPD) was optimized and established to simultaneously extract 13 bioactive compounds (7 coumarins and 6 phenolic acids) in Angelicae Pubescentis Radix (APR) by ultrahigh performance liquid chromatography coupled with photodiode array detector (UHPLC-PDA). Diol was chosen as the dispersing sorbent and methanol solution was used as the elution solvent. The preparation procedures for the MSPD including the types of sorbents, mass ratio of matrix to sorbent, grinding time, type, concentration and volume of elution solvent were investigated. Under the optimal conditions, good recoveries of the 13 target compounds were obtained in the range of 94.8–107% (RSD < 3.22%). The limits of detection (LODs) and limits of quantitation (LOQs) were in the ranges of 0.08–0.12 μg mL-1 and 0.16–0.24 μg mL-1, respectively. Compared with the traditional method, it was a green and environmentally friendly technique. The results proved that the established method was successfully applied to the extraction and determination of 13 target bioactive compounds for quality control in APR.neochlorogenic acid, chlorogenic acid, 4-dicaffeoylquinic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C,umbelliferae, columbianetin, imperatorin, osthole, isoimperatorin were obtained from Chengdu Desite Biological Technology Co., Ltd. (Chengdu, China).
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International journal of analytical chemistry-13 Feb 2019
An LC-MS/MS Method for Simultaneous Determination of the Toxic and Active Components of Cortex Periplocae in Rat Plasma and Application to a Pharmacokinetic Study期刊名：International journal of analytical chemistry文献编号：DOI 10.1155/2019/1639619文献地址：https://www.hindawi.com/journals/ijac/2019/1639619/发表日期：13 Feb 2019AbstractA sensitive and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determine the toxic and other active components including isovanillin, scopoletin, periplocin, periplogenin, and periplocymarin after oral administration of cortex periplocae extract to rats. Plasma samples were prepared by protein precipitation with methanol. All compounds were separated on a column with gradient elution using acetonitrile and formic acid aqueous solution (0.1%, v/v) as the mobile phase at a flow rate of 0.3 mL/min. The detection of all compounds was accomplished by multiple-reaction monitoring (MRM) in the positive electrospray ionization mode. The LC-MS/MS method exhibited good linearity for five analytes. The lower limit of quantification (LLOQ) was 0.48 ng/mL for scopoletin, periplogenin, and periplocymarin; 2.4 ng/mL for isovanillin and periplocin. The extraction recoveries of all compounds were more than 90% and the RSDs were below 10%. It was found that the absorption of scopoletin and periplocin was rapid in vivo after oral administration of cortex periplocae extract. Furthermore, periplocymarin possessed abundant plasma exposure. The results demonstrated that the validated method was efficiently applied for the pharmacokinetic studies of isovanillin, scopoletin, periplocin, periplogenin, and periplocymarin after oral administration of cortex periplocae extract.Periplocin (purity: 99.18%), periplocymarin (purity: 99.93%), periplogenin (purity: 99.81%), and scopoletin (purity ≥98%) were supplied from Chengdu Desite Biological Technology Co., Ltd. (Chengdu, China).
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Sustainable Chemistry and Pharmacy-26 March 2020
Ultrasound-enhanced matrix solid-phase dispersion micro-extraction applying Mesoporous Molecular Sieve SBA-15 for the determination of multiple compounds in Fructus Psoraleae期刊名：Sustainable Chemistry and Pharmacy文献编号：DOI 10.1016/j.scp.2019.100198文献地址：https://www.sciencedirect.com/science/article/pii/S2352554119302864?via%3Dihub发表日期：26 March 2020AbstractAn efficient ultrasound enhanced matrix solid-phase dispersion micro-extraction method was established for the extraction and determination of multiple ingredients (psoralenoside, isopsoralenoside, psoralen, isopsoralen, bavachin, psoralidin, isobavachalcone and bakuchiol) from Fructus Psoraleae applying ultra-high-performance liquid chromatography. The experimental factors affecting the extraction efficiency, including extraction solvent concentration, extraction solvent volume and ultrasound time, were optimized by the design of experiments methodology. Central composite design was applied in this optimization approach as a quadratic model. Under optimum conditions, good linear regression (r2 > 0.9999) and recovery (96.8%–104%, RSD<3.73%) for targets were attained. In addition, the intra-day of analytes was below 4.42% and inter-day precision of analytes was below 2.08%. This proposed method only required small amounts of reagent (0.5 mL) and materials usage (20 mg). Thus, the present study applied the efficient method to exhibit fast preparation and determination of eight ingredients of continents in Fructus Psoraleae.psoralen,psoralenoside, isopsoralen, isopsoralenoside, isobavachalcone, bavachin, bakuchiol and psoralidin were purchased from Chengdu Desite Biological Technology Co., Ltd (Chengdu, China)
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Journal of Separation Science-03 February 2018
Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid-phase dispersion extraction coupled with ultra high-performance liquid chromatography with photodiode array detection and quadrupole time-of-flight mass spectrometry期刊名：Journal of Separation Science文献编号：DOI 10.1002/jssc.201701411文献地址：https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201701411发表日期：03 February 2018
Abstract
A simple and effective sample preparation process based on miniaturized matrix solid‐phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5‐dicaffeoylqunic acid, 1,5‐dicaffeoylqunic acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol‐3‐O‐rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenanthraquinones (cryptotanshinone), and furans (5‐hydroxymethylfurfural) in Naoxintong capsule by ultra high‐performance liquid chromatography. The optimized condition was that 25 mg Naoxintong powder was blended homogeneously with 100 mg Florisil PR for 4 min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid‐phase dispersion coupled with ultra high‐performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid‐phase dispersion coupled with ultra high‐performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.Reference standards including gallic acid (GA), 5-hydroxymethylfurfural, chlorogenic acid, paeoniflorin, ferulic acid, 3,5-dicaffeoylqunic acid, 1,5-dicaffeoylqunic acid, kaempferol-3-O-rutinoside, rosmarinic acid, lithospermic acid, salvianolic acid B, calycosin, formononetin, ligustilide, butyllidephthalide, and cryptotanshinone were purchased from Chengdu Desite Biological Technology Co., Ltd (Chengdu, China)
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Molecules-23 July 2019
Identification of Metabolites of Eupatorin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS期刊名：Molecules文献编号：DOI 10.3390/molecules24142658文献地址：https://www.mdpi.com/1420-3049/24/14/2658发表日期：23 July 2019AbstractEupatorin is the major bioactive component of Java tea (Orthosiphon stamineus), exhibiting strong anticancer and anti-inflammatory activities. However, no research on the metabolism of eupatorin has been reported to date. In the present study, ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) combined with an efficient online data acquisition and a multiple data processing method were developed for metabolite identification in vivo (rat plasma, bile, urine and feces) and in vitro (rat liver microsomes and intestinal flora). A total of 51 metabolites in vivo, 60 metabolites in vitro were structurally characterized. The loss of CH2, CH2O, O, CO, oxidation, methylation, glucuronidation, sulfate conjugation, N-acetylation, hydrogenation, ketone formation, glycine conjugation, glutamine conjugation and glucose conjugation were the main metabolic pathways of eupatorin. This was the first identification of metabolites of eupatorin in vivo and in vitro and it will provide reference and valuable evidence for further development of new pharmaceuticals and pharmacological mechanisms.Eupatorin (855-96-9, purity > 98.94%) was purchased from Chengdu Desite Biological Technology Co., Ltd. (Chengdu, China)
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The Journal of Supercritical Fluids-26 August 2019
Solubility measurement and RESOLV-assisted nanonization of gambogic acid in supercritical carbon dioxide for cancer therapy期刊名：The Journal of Supercritical Fluids文献编号：DOI 10.1016/j.supflu.2019.04.008文献地址：https://www.sciencedirect.com/science/article/pii/S0896844619300658?via%3Dihub发表日期：26 August 2019AbstractDespite the captivating interest for its potential anticancer efficacy against multiple types of cancer and ability to overcome multidrug resistance, the practical application of gambogic acid (GA) has been hampered by its poor water solubility. Herein, the fabrication of nanosized GA particles (251.2 nm ± 85.6 nm) using eco-friendly supercritical fluid (SCF)-assisted rapid expansion of a supercritical solution into a liquid solvent (RESOLV) process was demonstrated. The equilibrium solubility of GA in supercritical carbon dioxide (SC−CO2) was initially determined at altered supercritical conditions (P: 10.0 MPa to 30.0 MPa, T: 308.15 K–328.15 K) and the obtained data points were then correlated using various density-based models including Méndez-Santiago and Teja, Bartle as well as Chrastil models. The solubility of GA in SC−CO2 was found to be between 1.63 × 10−6 and 22.62 × 10−6 in terms of GA mole fraction, and considerably good correlation agreement was obtained with all the applied models and being the lowest deviations obtained with the Méndez-Santiago and Teja model. Furthermore, the fabricated GA nanoparticles (NPs) through benign RESOLV technique, were systematically characterized. Subsequently, in vitro antiproliferation tests resulted in its augmented antitumor efficacy due to enhanced solubility of GA. Together, the results have suggested that the benign RESOLV process has successfully fabricated nanosuspensions of GA with enhanced bioavailability and antineoplastic efficacy and may have great potential in pharmaceutics.gambogic acid was purchased from DeSiTe Biological Technology Co. Ltd. (Chengdu, China)
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