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Royal Society of Chemistry-12 Aug 2019
A comprehensive study of eriocitrin metabolism in vivo and in vitro based on an efficient UHPLC-Q-TOF-MS/MS strategy
期刊名：Royal Society of Chemistry文献编号：DOI 10.1039/C9RA03037A文献地址：https://pubs.rsc.org/en/content/articlelanding/2019/RA/C9RA03037A#!divAbstract发表日期：12 Aug 2019AbstractEriocitrin, a main flavonoid in lemons, possesses strong antioxidant, lipid-lowering and anticancer activities and has long been used in food, beverages and wine. However, its metabolism in vivo and in vitro is still unclear. In this study, an efficient strategy was developed to detect and identify metabolites of eriocitrin by using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) based on online data acquisition and multiple data processing techniques. A total of 32 metabolites in vivo and 27 metabolites in vitro were obtained based on the above method. Furthermore, the main metabolic pathways of eriocitrin included reduction, hydrogenation, N-acetylation, ketone formation, oxidation, methylation, sulfate conjugation, glutamine conjugation, glycine conjugation, desaturation and demethylation to carboxylic acid. This study will lay a foundation for further studies on the metabolic mechanisms of eriocitrin.Eriocitrin (13463-28-0, purity > 98.5%) was purchased from Chengdu Desite Biological Technology Co. Ltd (Chengdu, China)
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Journal of chromatography. A-3 September 2019
A chitosan solution-based vortex-forced matrix solid phase dispersion method for the extraction and determination of four bioactive constituents from Ligustri Lucidi Fructus by high performance liquid chromatography期刊名：Journal of chromatography. A文献编号：DOI 10.1016/j.chroma.2019.460509文献地址：https://www.sciencedirect.com/science/article/pii/S0021967319308933?via%3Dihub发表日期：3 September 2019AbstractA simple and efficient sample preparation method to extract four bioactive compounds (echinacoside, specnuezhenide, oleuropein and nuezhenoside G13) from Ligustri Lucidi Fructus was established by vortex-forced matrix solid phase dispersion (VFMSPD) method. Chitosan solution was applied as green eluent in this procedure and Celite AZO was employed as dispersant. High performance liquid chromatography (HPLC) equipped with ultraviolet (UV) detector was used to analyze the target analytes. The best result of the investigation was obtained with Celite AZO as dispersant, sample/ dispersant ratio as 1:1, grinding for 2 min, 1 mL high-viscosity chitosan solution (0.5 mg mL−1) used as the elution reagent and vortex mixing for 1.5 min. The method exhibit a good linearity for the analytes (r2 > 0.999). The absolute recoveries of the four target compounds in Ligustri Lucidi Fructus ranged from 90.7% to 98.8% and the relative recoveries of the target compounds ranged from 99.2% to 102% (RSD ≤ 3.4%), which were obtained by the final optimization method. Consequently, the newly developed chitosan solution-based vortex-forced matrix solid phase dispersion (MSPD) combined with HPLC could be efficiently applied to extract and analyze the target compounds in Ligustri Lucidi Fructus samples.Standard compounds of specnuezhenide, oleuropein, echinacoside and nuezhenoside G13 (the chemical structures were displayed in Fig. 1) were provided by Chengdu Desite Biological Technology Co. Ltd. (Chengdu, China)
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Evidence-Based Complementary and Alternative Medicine
Pharmacokinetic and Bioavailability Studies of Embelin after Intravenous and Oral Administration to Rats期刊名：Evidence-Based Complementary and Alternative Medicine文献编号：DOI 10.1155/2019/9682495文献地址：https://www.hindawi.com/journals/ecam/2019/9682495/发表日期：20 Mar 2019AbstractEmbelin exhibits the broad bioactivities such as antitumor, antifertility, antidiabetic, anti-inflammatory, antioxidant, anticonvulsant, anxiolytic, antimicrobial, and hepatoprotective activity. In order to further understand the pharmacokinetic characteristics and oral bioavailability of embelin in vivo, the concentration of embelin in rat plasma was determined by a sensitive high-performance liquid chromatography with diode array detector (HPLC-DAD). The preparation of samples was accomplished by a simple precipitating protein with methanol. Emodin was selected as the internal standard (IS). Embelin and IS were completely separated on an analytical column (Extend-C18, 4.6 × 250 mm, 5 μm) using 0.1% phosphoric acid in methanol and 0.1% phosphoric acid in aqueous solution (90:10, v/v) as the mobile phase. The lower limit of quantification was 0.15 μg/mL. Oral bioavailability of embelin was 30.2 ± 11.9%. This study could provide the information about pharmacokinetics and oral bioavailability of embelin, which was useful to assess the clinic efficacy and safety and promote further development of embelin.Emodin was purchased from Chengdu Desite Biological Technology Co., Ltd. (Chengdu, China)
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Frontiers in pharmacology-09 January 2019
Simultaneous Extraction and Determination of Compounds With Different Polarities From Platycladi Cacumen by AQ C18-Based Vortex-Homogenized Matrix Solid-Phase Dispersion With Ionic Liquid期刊名：Frontiers in pharmacology文献编号：DOI 10.3389/fphar.2018.01532文献地址：https://link.springer.com/article/10.1007%2Fs13318-017-0444-8发表日期：09 January 2019 This study presented a rapid, simple and environmentally friendly method of employing AQ C18-based vortex-homogenized matrix solid-phase dispersion with ionic liquid (AQ C18-IL-VHMSPD) for the extraction of compounds with different polarities from Platycladi Cacumen (PC) samples by ultra high-performance liquid chromatography with PDA detection. AQ C18 (aqua C18) and ionic liquid ([Bmim]BF4) were used as the adsorbent and green elution reagent in vortex-homogenized MSPD procedure. The AQ C18-IL-VHMSPD conditions were optimized by studying several experimental parameters including the type of ionic liquid, the type of adsorbent, ratio of sample to adsorbent, the concentration and volume of ionic liquid, grinding time and vortex time. The recoveries of the target compounds were in the range of 96.9–104% with relative standard deviation values no more than 2.8%. The limits of detection and limits of quantitation were in the range of 0.2–1.2 and 1.0–5.4 ng mL-1, respectively. Compared with the traditional ultrasonic-assisted extraction, the developed AQ C18-IL-VHMSPD method required less sample, reagent and time. It was concluded that the AQ C18-IL-VHMSPD method was a powerful method for the extraction and quantification of the high polarity and low polarity compounds in traditional Chinese medicines samples.Reference standards of myricitrin, isoquercitrin, quercitrin,amentoflavone and hinokiflavone were purchased from Chengdu Desite Biological Technology Co., Ltd (Chengdu, China)
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Identification and Quality Evaluation of Raw and Processed Asarum Species Using Microscopy, DNA Barcoding, and Gas Chromatography-Mass Spectrometry
Asarum (Aristolochiaceae) is one of the common herbs used to relieve exterior syndromes. Some volatile components of Asarum which have toxic effect may cause adverse reactions such as headache, general tension, unconsciousness, and respiratory paralysis. Therefore, Asarum is normally processed to reduce such toxicity and adverse effects. The bioactive ingredients contained in different Asarum herbs vary significantly; this variation may be attributed to their differences in species, origins, or processing methods. In this study, 16 batches of Asarum herbs were collected, and their species were identified using DNA barcoding, which is a method for distinguishing plant species, coupled with microscopy. A gas chromatography-mass spectrometry (GC-MS) method for simultaneous determination of 10 compounds was established to evaluate the contents of raw and processed Asarum herbs. Multivariate analysis was then applied to compare different batches of herbs based on the GC-MS data. DNA barcoding identified the herbs as being derived from four sources, and herbs from different origins showed different microscopic features. The results demonstrated that most of the samples were clearly clustered into distinct groups that corresponded to species types. All raw and processed samples were classified by partial least squares discriminant analysis (PLS-DA) based on the 10 analyzed compounds. The findings suggested that safrole and methyleugenol with a variable importance in the project (VIP) > 1 are unique compounds that can be used to differentiate between Asarum species. Safrole, methyleugenol, and 2,6,6-trimethylcyclohepta-2,4-dien-1-one were identified as significant constituents, the presence of which can be used to differentiate between raw and processed Asarum samples. These results indicate that species and processing methods show important effects on the composition of Asarum herbs.
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Chemicals. Reference standards of (1R)-(+)-alpha-pinene, (+)-car-3-ene, cineole, (−)-β-pinene, (−)-borneol, 2,6,6-trimethylcyclohepta-2,4-dien-1-one, α-terpineol, estragole, safrole, and methyleugenol were purchased from Chengdu Desite Biological Technology Co. Ltd. (Sichuan, China)
原文地址：https://www.hindawi.com/journals/jamc/2020/2690238/
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International Journal of Phytotherapy and Phytopharmacology
AbstractBackgroundIt is of utmost significance to choose the bioactive components as quality markers for ensuring the effectiveness of traditional Chinese medicine. Nonetheless, some markers are able to assess effectively the quality of TCM without considering the pharmacological mechanisms and intrinsic chemical complexities. ......
ObjectiveThis underscores the need to discover new and efficient markers which can assess both quality and mechanism of action. Herein, a strategy of bioactive-chemical quality markers combination was proposed to improve the level of the quality control of TCM by metabolomics coupled with chemometrics.......
Reference standards of typhaneoside, kaempferol-3-O-neohesperidoside, citric acid, linolenic acid, kaempferol-3-O-neohesperidoside, astragalin, naringenin, puerarin, and succinic acid with 98% purity on the basis of HPLC analysis were supplied by Chengdu Desite Biological Technology Co. Ltd. (Sichuan, China)
原文地址：https://www.sciencedirect.com/science/article/abs/pii/S0944711320300775?via%3Dihub
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